Metabolomics is a now a long established method of examination of analytical chemistry. It relies on a variety of statistical and multi-variant technologies for both information mining and for interpreting the data.
What Is Metabolomics ?
The technique of metabolomics is the identification and quantification of the complete set of metabolites in a biological system.
It demands the quantitative measurement of the dynamic metabolite response of a living organism to genetic modification and to various physiological stimuli. It includes all the chemical compounds that are small molecules and which we term as metabolites such as amino acids, lipids, amino acids and various phenolic compounds. Most importantly though, it excludes the proteins and peptides.
The objective is to obtain important insights into physiological and disease states. It helps us understand in depth the various underlying biochemical pathways.
There are many different formats within the study of metabolomics. The most wide ranging is untargeted. This measures many compounds including their ratios but is least accurate. The next level is metabolic profiling which looks at a set of related compounds. The most accurate is a targeted analysis which measures and quantifies specific compounds.
The technique relies primarily on chromatography. These are separation techniques. They inlcude gas chromatography, capillary electrophoresis, high performance liquid chromatography (HPLC) and ultra-performance liquid chromatography (UPLC).