The glutamine synthetase (GS) expression system
The glutamine synthetase (GS) expression system is one of the most widely used and industrially proven platforms for the development of Chinese hamster ovary (CHO) […]
Biotechnology
What 3-Hydroxypropionic Acid (3-HP) is and why it matters
3-Hydroxypropionic acid (3-HP; chemical formula C₃H₆O₃) is a three-carbon hydroxycarboxylic acid with a hydroxyl group on C-3 and a terminal carboxyl group. It is a […]
The Eukaryotic Cell Cycle: Stages, DNA Dynamics, and Regulatory Mechanisms
The cell cycle is the fundamental process by which cells grow, duplicate their genetic material, and divide to form two daughter cells. This cycle underpins […]
How Are New Genes Created?
New genes are created through a variety of molecular mechanisms that introduce genetic novelty into genomes over evolutionary time. Here are the main ways new […]
The T4 Bacteriophage- A Model of Molecular Precision
Bacteriophages, viruses that infect bacteria, are among the most abundant biological entities on Earth. Among these, the T4 bacteriophage (commonly referred to as the T4 […]
Halophytic Bacteria and Their Applications in Biotechnology
Introduction Halophytic bacteria, also known as halophiles, are microorganisms that thrive in high-salt environments, often exceeding the salinity of seawater. These extremophiles are typically found […]
Fusion Tags: Benefits And Challenges
FoodWrite talked about affinity tags not so long ago well here we talk about fusion tags which include the family of affinity tags. What Are […]
Polyethyleneimine (PEI) and its use in Biotechnology
Polyethyleneimine (PEI) is a versatile and widely used polymer in the bioprocess and biotechnology industries, primarily due to its polycationic (positively charged) nature, which allows […]
Lipid Nanoparticles
Lipid nanoparticles (LNPs) are nanoscale delivery vehicles made primarily of lipids that self-assemble into stable particles. They are widely used for delivering nucleic acids like […]
Northern Blotting
Northern blotting is a fundamental molecular biology technique used to detect and quantify specific RNA molecules within a complex mixture of RNA extracted from cells […]
The Production of Recombinant Proteins Using Virus-Free Methods
Recombinant proteins are proteins encoded by recombinant DNA that has been cloned in an expression system allowing gene expression and protein production. These proteins are […]
Lentiviruses and Lentiviral Vectors (LVs): Biology and Applications in Biotechnology and Medicine
Introduction Lentiviruses are a genus of viruses within the family Retroviridae, characterized by their ability to integrate into the host genome and establish persistent infections. […]
What is Perfusion N-1 Culturing?
The N-1 perfusion method is a bioprocessing technique used in upstream biomanufacturing, particularly in the production of therapeutic proteins such as monoclonal antibodies (mAbs) in […]
Cell-Free Protein Synthesis (CFPS): A Biotechnology Method for Protein Production
Introduction Cell-free protein synthesis (CFPS) represents a groundbreaking biotechnology platform for producing proteins without the need for living cells. Unlike traditional in vivo protein expression […]
Recessive Dystrophic Epidermolysis Bullosa (RDEB)
RDEB is a severe, inherited skin disorder caused by loss-of-function mutations in the COL7A1 gene, which encodes type VII collagen, a structural protein that forms […]
Bacillus clausii: A Resilient Probiotic with Diverse Applications
Bacillus clausii is a Gram-positive, rod-shaped, spore-forming bacterium widely recognized for its probiotic properties. Originally isolated from soil, it has gained significant attention in clinical, […]
Bacillus coagulans: A Versatile Probiotic Bacterium
Bacillus coagulans is a spore-forming, lactic acid-producing bacterium that has gained considerable attention in the fields of microbiology, nutrition, and therapeutics. Though often grouped with […]
Ageing Mechanisms In Yeasts
To understand the mechanisms of ageing in Saccharomyces cerevisiae (budding yeast), a variety of experimental and computational approaches can be used, targeting the two main […]
Southern Blotting – Principles, Methodology, and Applications
Southern blotting is a molecular biology technique developed by Edwin Southern in 1975, designed to detect specific DNA sequences within a complex mixture of DNA. […]
Gotta Deal With Microplastics
The biotechnology of dealing with microplastics—tiny plastic particles less than 5mm in size—is an emerging and rapidly developing field. It focuses on biological solutions to […]