What is Perfusion N-1 Culturing?
The N-1 perfusion method is a bioprocessing technique used in upstream biomanufacturing, particularly in the production of therapeutic proteins such as monoclonal antibodies (mAbs) in […]
Biotechnology
Cell-Free Protein Synthesis (CFPS): A Biotechnology Method for Protein Production
Introduction Cell-free protein synthesis (CFPS) represents a groundbreaking biotechnology platform for producing proteins without the need for living cells. Unlike traditional in vivo protein expression […]
Bacillus coagulans: A Versatile Probiotic Bacterium
Bacillus coagulans is a spore-forming, lactic acid-producing bacterium that has gained considerable attention in the fields of microbiology, nutrition, and therapeutics. Though often grouped with […]
Ageing Mechanisms In Yeasts
To understand the mechanisms of ageing in Saccharomyces cerevisiae (budding yeast), a variety of experimental and computational approaches can be used, targeting the two main […]
Southern Blotting – Principles, Methodology, and Applications
Southern blotting is a molecular biology technique developed by Edwin Southern in 1975, designed to detect specific DNA sequences within a complex mixture of DNA. […]
Gotta Deal With Microplastics
The biotechnology of dealing with microplastics—tiny plastic particles less than 5mm in size—is an emerging and rapidly developing field. It focuses on biological solutions to […]
Neurospora crassa
Neurospora crassa is a filamentous fungus that has been extensively used as a model organism in genetics, molecular biology, and biochemistry. It’s also involved in […]
Metabolic Flux: What Is It?
What is Metabolic Flux? Metabolic flux refers to the rate at which metabolites flow through a metabolic pathway in a cell. It’s typically measured in […]
The Yeast: Kluyveromyces lactis
Kluyveromyces lactis is a yeast species with some pretty awesome biotech uses, especially in the dairy and enzyme production industries. It’s kind of a workhorse […]
Adenosine Triphosphate (ATP) Regeneration
In bioengineering and life sciences, adenosine triphosphate (ATP) regeneration is a crucial aspect of designing cell-free systems, metabolic engineering, biosensors, and biocatalytic processes. Since ATP […]
Cockayne Syndrome (CS)
Cockayne Syndrome (CS) is a rare, autosomal recessive genetic disorder characterized by growth failure, premature aging, progressive neurodegeneration, and sensitivity to sunlight (though not to […]
Xeroderma pigmentosum
Xeroderma pigmentosum (XP) is a rare, inherited genetic disorder characterized by an extreme sensitivity to ultraviolet (UV) light, leading to severe skin damage, early onset […]
Combinatorial Gene Control
Combinatorial gene control refers to the regulation of gene expression by the coordinated action of multiple gene regulatory proteins, such as transcription factors and co-regulators, […]
The Alternative Cassette Exon
An alternative cassette exon (also known as skipped exon) is one of the most common forms of alternative splicing. In this process, a specific exon […]
Alternative Splicing
Alternative splicing is a key process in eukaryotic gene expression, allowing a single gene to produce multiple protein isoforms. This process occurs during the maturation […]
The Power of Optogenetic Tools in Microbial Control
Optogenetic tools have revolutionized the way we study and control biological systems by using light to precisely manipulate cellular processes (Wang et al., 2025). When […]
Deep Learning for Sequence-to-Expression Models in Strain Optimization
Deep learning is a subset of machine learning that uses neural networks with multiple layers i.e. deep neural networks, to learn complex patterns from large […]
What is RNA (Ribonucleic Acid)?
RNA (Ribonucleic Acid) is a molecule essential to many biological processes, acting primarily as a messenger and functional molecule in cells. While it shares some […]
What is Non-Coding RNA (ncRNA)?
Non-coding RNA (ncRNA) refers to RNA molecules that are transcribed from DNA but do not encode proteins. Instead, they perform a variety of regulatory, structural, […]
Getting To Grips With Cryopreservation In The Food And Biotechnology Industry
Cryopreservation refers to the process of preserving biological materials at extremely low temperatures to halt all biochemical processes, including metabolism and enzymatic activity. It plays […]