Polyethyleneimine (PEI) and its use in Biotechnology
Polyethyleneimine (PEI) is a versatile and widely used polymer in the bioprocess and biotechnology industries, primarily due to its polycationic (positively charged) nature, which allows […]
Biotechnology
Lipid Nanoparticles
Lipid nanoparticles (LNPs) are nanoscale delivery vehicles made primarily of lipids that self-assemble into stable particles. They are widely used for delivering nucleic acids like […]
Northern Blotting
Northern blotting is a fundamental molecular biology technique used to detect and quantify specific RNA molecules within a complex mixture of RNA extracted from cells […]
The Production of Recombinant Proteins Using Virus-Free Methods
Recombinant proteins are proteins encoded by recombinant DNA that has been cloned in an expression system allowing gene expression and protein production. These proteins are […]
Fermentation Control: AI And ML In Device Control
Artificial intelligence (AI) and machine learning (ML) have become increasingly influential in device control across industrial processes, and fermentation is one of the domains where […]
Lentiviruses and Lentiviral Vectors (LVs): Biology and Applications in Biotechnology and Medicine
Introduction Lentiviruses are a genus of viruses within the family Retroviridae, characterized by their ability to integrate into the host genome and establish persistent infections. […]
What is Perfusion N-1 Culturing?
The N-1 perfusion method is a bioprocessing technique used in upstream biomanufacturing, particularly in the production of therapeutic proteins such as monoclonal antibodies (mAbs) in […]
Cell-Free Protein Synthesis (CFPS): A Biotechnology Method for Protein Production
Introduction Cell-free protein synthesis (CFPS) represents a groundbreaking biotechnology platform for producing proteins without the need for living cells. Unlike traditional in vivo protein expression […]
Recessive Dystrophic Epidermolysis Bullosa (RDEB)
RDEB is a severe, inherited skin disorder caused by loss-of-function mutations in the COL7A1 gene, which encodes type VII collagen, a structural protein that forms […]
Fermentation Control: Using AI and ML In Deviation Detection
Artificial intelligence (AI) and machine learning (ML) have become central tools for deviation detection in modern fermentation processes, particularly as industrial biotechnology has shifted toward […]
How does synthetic data and simulations try to address gaps in our understanding of biotechnology?
Synthetic data and simulations are used in biotechnology to partially compensate for real-world data limitations, not to replace biology. They aim to give AI more […]
Bacillus clausii: A Resilient Probiotic with Diverse Applications
Bacillus clausii is a Gram-positive, rod-shaped, spore-forming bacterium widely recognized for its probiotic properties. Originally isolated from soil, it has gained significant attention in clinical, […]
Bacillus coagulans: A Versatile Probiotic Bacterium
Bacillus coagulans is a spore-forming, lactic acid-producing bacterium that has gained considerable attention in the fields of microbiology, nutrition, and therapeutics. Though often grouped with […]
Ageing Mechanisms In Yeasts
To understand the mechanisms of ageing in Saccharomyces cerevisiae (budding yeast), a variety of experimental and computational approaches can be used, targeting the two main […]
Southern Blotting – Principles, Methodology, and Applications
Southern blotting is a molecular biology technique developed by Edwin Southern in 1975, designed to detect specific DNA sequences within a complex mixture of DNA. […]
Gotta Deal With Microplastics
The biotechnology of dealing with microplastics—tiny plastic particles less than 5mm in size—is an emerging and rapidly developing field. It focuses on biological solutions to […]
Neurospora crassa
Neurospora crassa is a filamentous fungus that has been extensively used as a model organism in genetics, molecular biology, and biochemistry. It’s also involved in […]
Metabolic Flux: What Is It?
What is Metabolic Flux? Metabolic flux refers to the rate at which metabolites flow through a metabolic pathway in a cell. It’s typically measured in […]
The Yeast: Kluyveromyces lactis
Kluyveromyces lactis is a yeast species with some pretty awesome biotech uses, especially in the dairy and enzyme production industries. It’s kind of a workhorse […]
Lipase-Mediated Enantioselective Esterification
Lipase-mediated enantioselective esterification is a cornerstone methodology in modern biocatalysis and asymmetric synthesis, widely applied in the preparation of optically active compounds for pharmaceuticals, agrochemicals, […]