The volatile constituents were extracted with ethanol from the roots and aerial parts of Hierochloe odorata L. var. pubescens Kryl. growing in the east of China. These  were examined by a combination of gas chromatography (GC), gas chromatography-mass spectrometry (GC-MS) and high pressure liquid chromatography (HPLC) techniques. A total of 169 volatile compounds were identified. This volatile oil was rich in coumarin, which accounted for 10.3 % of the root oil and 24.9% of the aerial part oil. Quantitative analysis by HPLC showed that ethanol extract of the roots to contain 3.57% (m/m) of coumarin and that of the aerial parts 3.72% (m/m).

Reference

Ueyama, Y., Arai, T., Hashimoto, S. (1991), Volatile constituents of ethanol extracts of Hierochloe odorata L. var. Pubescens kryl. Flavour Fragr. J., 6 pp. 63–68. doi: 10.1002/ffj.2730060108